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The Carboniferous volcanic reservoir in the Chepaizi Uplift became an exploration hot target in recent years for its substantial amount of oils discovered. However, most of the Carboniferous heavy oils were biodegraded to PM7 or higher with orders of magnitude variation in oil viscosities. Two oil groups (I and II) exactly corresponding to the western and eastern Chepaizi Uplift were distinguished according to their source diagnose. Furthermore, three oil families (II1, II2 and II3), with the biodegradation level of PM7, PM8–8+, PM9+, respectively, were classified based on molecular compositions and parameter-stripping method of strongly bioresistant parameters. Allowing for this extremely high biodegradation case, more biodegradation refractory compound class were added to establish a refined Manco scale to quantitatively evaluate the biodegradation extent. Refined Manco number (RMN2) positively correlated with the oil density, NSO contents, and absolute concentrations of diasteranes and gammacerane, negatively correlated with the absolute concentrations of diahopane, summed tricyclic terpanes and pentacyclic terpanes. This refined scale showed higher resolution than the PM one to differentiate the biodegradation extent of Carboniferous heavy oils from the Chepaizi Uplift, especially those with same PM values but different oil viscosities.